Biochemical Studies on the Protein Synthesis

Biochemical Studies on Amphibian Metamorphosis I. The effect of thyroxine on protein synthesis in the tadpole

Thyroxine has been shown to accelerate the synthesis of carbamyl phosphate synthetase in the liver of Rana catesbdana. Stimulation of carbamyl phosphate synthetase synthesis by thyroxine appears to be relatively specific because of the following observations: (I) succinoxidase activity decreased during the time that carbamyl phosphate synthetase increased; (2) liver catalase responded more slow...

Biochemical Studies on Amphibian Metamorphosis I. The effect of thyroxine on protein synthesis in the tadpole

Thyroxine has been shown to accelerate the synthesis of carbamyl phosphate synthetase in the liver of Rana catesbdana. Stimulation of carbamyl phosphate synthetase synthesis by thyroxine appears to be relatively specific because of the following observations: (I) succinoxidase activity decreased during the time that carbamyl phosphate synthetase increased; (2) liver catalase responded more slow...

Biochemical and Structural Studies on the Adaptor Protein p130Cas

Studies on Protein Synthesis in Vitro

The release of radioactive amino acid from the particulate fractions separated from the prelabelled livers of rats by centrifugation has been studied under various conditions. Although pure fractions may not have been obtained, great differences in behavior were observed. In the mitochondria and nuclei dinitrophenol (10(-4)M) causes an inhibition of release, but in microsomes the opposite effec...

Biochemical studies of mammalian oogenesis: Protein synthesis during oocyte growth and meiotic maturation in the mouse.

Using oocytes isolated from juvenile and adult mice, we have examined the qualitative patterns of protein synthesis during growth and during meiotic maturation of these oocytes. Oocytes were cultured in a denned medium in the presence of [^Slmethionine and radioactively labelled proteins were separated by SDS-polyacrylamide gel electrophoresis and detected by fluorography. The results of these ...